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Tau, phosphorylated (Ser422) (Tau Protein, Microtubule-associated Protein, MAP)

Cat no: T1032-74C

Tau, phosphorylated (Ser422) (Tau Protein, Microtubule-associated Protein, MAP)

Tau is a microtubule-associated phosphoprotein (MAP), localized in neuronal axons. It promotes tubulin polymerization and stabilizes microtubules. Tau proteins constitute a family of six isoforms, which ranging from 352 to 441 amino acids. The tau variants differ from each other by the presence of either three or four repeat-regions in the carboxy-terminal part of the molecule and the absence or presence of one or two inserts in the amino-terminal portion.\nTau is hyperphosphorylated by ERK, GSK-3, TPKII, and CDK5. At least thirty phosphorylation sites have been described, including Thr39, Ser46, Thr50, Thr69, Thr153, Thr175, Thr181, Ser198, Ser199, Ser202, Thr205, Ser208, Ser210, Thr212, Ser214, Thr217, Thr231, Ser235, Ser237, Ser241, Ser262, Ser285, Ser305, Ser324, Ser352, Ser356, Ser396, Ser400, Thr403, Ser404, Ser409, Ser412, Ser413, Ser416, and Ser422. Specifically, TPKII phosphorylates serines 202 and 404. GSK-3b transfection phosphorylates serines 199, 202, 235, 396, 404, and 413, and threonines 205 and 231. These sites are among the major abnormal phosphorylation sites of tau. Phosphorylation on these sites reduces the ability of a given tau species to promote microtubule self-assembly. Hyperphosphorylated tau is the major protein of the paired helical filaments (PHFs), which make up the pathological neurofibrillary tangles of Alzheimer

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SPECIFICATIONS

Catalog Number

T1032-74C

Size

40ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, pH 7.4, 0.02% sodium azide.Reconstitution:

P Type

Pab

Purity

Purified by Affinity chromatography.

Isotype

IgG

Additional Info

Recognizes human tau only when phosphorylated at serine 422.

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