Tumor Necrosis Factor Alpha (TNFa) BioAssay(TM) ELISA Kit (Rabbit)

Tumor Necrosis Factor Alpha (TNFa) BioAssay(TM) ELISA Kit (Rabbit) is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNFa in rabbit serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 3.12-200pg/ml Sensitivity: 1.25pg/ml Intra-Assay CV: < 10% Inter-Assay CV: < 12% Kit Components: *028668A: Microtiter Strips, 1x96 wells, Pre-coated; ready to use *028668B: Standard, 2x1vial 028668C: Standard Diluent, 1x20ml *028668D: Detection Reagent A, 1x120ul *028668E: Detection Reagent B, 1x120ul 028668F: Assay Diluent A,1x12ml 028668G: Assay Diluent B, 1x12ml 028668H: TMB Substrate, 1x9ml 028668K: Stop Solution, 1x6ml 028668L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (028668K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *028668A, *028668B, *028668D and *028668E at -20 degrees C. Store all the other components at 4 degrees C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degrees C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degrees C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degrees C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37 degrees C. 8. Add 50ul Stop Solution. Read at 450nm immediately.