Tumor Necrosis Factor Alpha (TNFa) BioAssay(TM) ELISA Kit (Rabbit)

Tumor Necrosis Factor Alpha (TNFa) BioAssay(TM) ELISA Kit (Rabbit) is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNFa in rabbit serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\n\nDetection Range:\n3.12-200pg/ml\n\nSensitivity:\n1.25pg/ml\n\nIntra-Assay CV: \n< 10%\n\nInter-Assay CV: \n< 12%\n\nKit Components:\n*028668A: Microtiter Strips, 1x96 wells, Pre-coated; ready to use\n*028668B: Standard, 2x1vial \n028668C: Standard Diluent, 1x20ml\n*028668D: Detection Reagent A, 1x120ul \n*028668E: Detection Reagent B, 1x120ul \n028668F: Assay Diluent A,1x12ml\n028668G: Assay Diluent B, 1x12ml\n028668H: TMB Substrate, 1x9ml\n028668K: Stop Solution, 1x6ml\n028668L: Wash Buffer, 30X, 1x20ml \n\nPrecaution:\nThe Stop Solution (028668K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.\n\nStorage and Stability:\nStore *028668A, *028668B, *028668D and *028668E at -20 degrees C. Store all the other components at 4 degrees C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.\n\nAssay Procedure Summary:\n1. Prepare all reagents, samples and standards.\n2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degrees C.\n3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degrees C.\n4. Aspirate and wash 3 times.\n5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degrees C.\n6. Aspirate and wash 5 times.\n7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37 degrees C.\n8. Add 50ul Stop Solution. Read at 450nm immediately.

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