Ubiquitin (FLJ25987, MGC8385, Polyubiquitin B, RPS27A, UBA52, UBA80, UBC, UBCEP1, UBCEP2, Ubiquitin B, UBB)

Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has an ATP dependent role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.\n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Frozen, paraffin): 1:50-1:100. Use ABC method. Incubate for 30-60min at RT.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

U1000-01N

Size

500ul

Applications

IHC

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as liquid, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Mayer RJ et al. Ubiquitin and dementia. Nature 340:193 (1989). <PUBMED:2547162> 2. Lennox G et al. Ubiquitin is a component of neurofibrillary tangles in a variety of neurodegenerative diseases. Neurosci Lett 94:211-7 (1988). <PUBMED:2853854> 3. Perry G et al. Ubiquitin is detected in neurofibrillary tangles and senile plaque neurites of Alzheimer disease brains. Proc Natl Acad Sci U S A 84:3033-6 (1987). <PUBMED:3033674>

Additional Info

Recognizes bovine ubiquitin. It also stains with the periphery of senile plaques and neurofibrillay tangles in Alzheimer's disease. Species Crossreactivity: human, rat, mouse.