UCHL1 (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1, UCH-L1, Neuron Cytoplasmic Protein 9.5, PGP 9.5, PGP9.5, Ubiquitin Thioesterase L1)

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1), a thiol protease belonging to the peptidase C12 family, is involved in the processing of ubiquitin precursors and of ubiquitinated proteins. UCH-L1 recognizes and hydrolyzes peptide bonds at the C-terminal glycine of ubiquitin. UCH-L1 is expressed in neurons and cells of the diffuse neuroendocrine system and thier tumors. Defects in UCH-L1 are the cause of Parkinson disease type 5.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nPC12 cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029638

Size

100ug

Applications

WB

Hosts

Ch/Bird

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.05% sodium azide, 10% glycerol.

P Type

Pab

Purity

Purified by IgY fraction.

References

1. Hillier, LW., et al. (2005). Nature. 434(7034): 724-731. 2. Choi, J., et al. (2004). J Biol Chem. 279:13256-13264. 3. Leroy, E., et al. (1998). Nature. 395 (6701):451-452.

Additional Info

Recognizes human UCH-L1. Species Crossreactivity: Mouse and rat.