URM1 (Ubiquitin-related Modifier 1, YIL008W)

Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Urm1 is a newly identified ubiquitin related modifier. Urm 1 is a 99aa protein terminated with glycine-glycine. Target proteins are conjugated to Urm1 via its C-terminal glycine. Initially Urm1 forms a thioester with a novel E1-like protein, Uba4.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:2000-1:10,000\nWestern Blot: 1:2000-1:10,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile dH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

168579

Size

500ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Yeast/Fun

Form

Supplied as a lyophilized powder in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide. Reconstitute with sterile dH2O or PBS.

P Type

Pab

Purity

Purified by Ion Exchange chromatography.

Isotype

IgG

Additional Info

Recognizes yeast Urm1.

Alternative Names

C9orf74 antibody, Chromosome 9 open reading frame 74 antibody, MGC2668 antibody, RP11 339B21.4 antibody, Ubiquitin Related Modifier 1 antibody, Ubiquitin related modifier 1 homolog antibody, Urm 1 antibody