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Urokinase Plasminogen Activator (uPA)

Cat no: U1820-11C


Supplier: United States Biological
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Urokinase plasminogen activator (uPA) is a serine protease. It converts the abundant proenzyme plasminogen to active plasmin and plays a key role in cancer invasion and a variety of tissue remodelling processes such as wound healing, mammary gland involution and placental development. Elevated levels of uPA are associated with poor prognosis in many types of cancer. Applications: Suitable for use in ELISA, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:50-1:100. Frozen sections & paraffin sections. Pretreatment with 0.05% Pronase E for 20 minutes. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: U1820-11C
Hosts: Goat
Applications: ELISA, Immunohistochemistry
Size: 20ul
Form: Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute in ddH2O.
P type: Pab
Purity: Serum
Additional info: Recognizes Human Urokinase plasminogen activator. There were no cross reactivities obtained with human tPA and human PAI-2.

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