Urokinase plasminogen activator (uPA) is a serine protease. It converts the abundant proenzyme
plasminogen to active plasmin and plays a key role in cancer invasion and a variety of tissue
remodelling processes such as wound healing, mammary gland involution and placental development. Elevated levels of uPA are associated with poor prognosis in many types of cancer.
Applications:
Suitable for use in ELISA, Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry: 1:50-1:100. Frozen sections & paraffin sections. Pretreatment with
0.05% Pronase E for 20 minutes.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.