USP9X (Probable Ubiquitin Carboxyl-Terminal Hydrolase FAF-X, Ubiquitin Thioesterase FAF-X, Ubiquitin-specific-processing Protease FAF-X, Deubiquitinating Enzyme FAF-X, Ubiquitin-specific Protease 9, X Chromosome, Fat fAcets Protein-related, X-linked, Fat

Protein ubiquitination and deubiquitination is a reversible process catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs) (1,2). DUBs are categorized into five subfamiles-USP, UCH, OTU, MJD, and JAMM. USP9X/hFAM is an X-linked member of the ubiquitin-specific protease (USP) subfamily of DUBs and possesses a well-conserved catalytic domain with cysteine peptidase activity, which allows for cleavage of ubiquitin and polyubiquitin conjugates. USP9X is the mammalian homolog of the Drosophila fat-facets (faf) gene, which is essential for normal eye development and viability of the early fly embryo (3,4). While USP9X expression is also critical for normal mammalian development (5-7), many of its substrates are only beginning to be elucidated. There is mounting evidence that USP9X functions in the formation of cell-cell contacts during the polarization of epithelial cells, in part through deubiquitination-dependent stabilization of molecules involved in maintaining the integrity of both adherens and tight junctions. Indeed, USP9X has been found to associate with AF-6, the b-catenin-E-cadherin complex, and EFA6 (8-11). Studies have also demonstrated that USP9X is an integral component of the TGF-b/BMP signaling cascade and regulates TGF-b responsiveness by opposing TRIM33-mediated monoubiquitination of SMAD4 (12). Recently, USP9X was shown to be overexpressed in a variety of human cancers, which was linked to enhanced cell survival and chemoresistance through its ability to deubiquitinate and stabilize the Mcl-1 oncoprotein. This evidence supports USP9X as a therapeutic target in the context of human malignancies such as follicular lymphomas and diffuse large B-cell lymphomas (13).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

U4099-67A

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Can, NHP

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

References

1.Nijman, S.M. et al. (2005) Cell 123, 773-86. 2.Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613. 3.Huang, Y. et al. (1995) Science 270, 1828-31. 4.Huang, Y. and Fischer-Vize, J.A. (1996) Development 122, 3207-16. P5.antaleon, M. et al. (2001) Mech Dev 109, 151-60. 6.Noma, T. et al. (2002) Mech Dev 119 Suppl 1, S91-5. 7.Xu, J. et al. (2005) J Neurosci Res 80, 47-55. 8.Taya, S. et al. (1998) J Cell Biol 142, 1053-62. 9.Taya, S. et al. (1999) Genes Cells 4, 757-67. 10.Murray, R.Z. et al. (2004) Mol Biol Cell 15, 1591-9. 11.Th

Additional Info

Recognizes endogenous levels of total human USP9X. Species Crossreactivity: mouse, rat, monkey and canine.