V5 Epitope Tag GKPIPNPLLGLDST (Pk-tag, Paramyxovirus, SV5)

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence.\n\nApplications:\nSuitable for use in ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot, and RIA.Other applications have not been tested. \n\nRecommended Dilution:\nELISA: 1:1000-1:5000\nWestern Blot: 1:1000-1:5000\nImmunohistochemistry: frozen\nOptimal dilutions to be determined by the researcher. \n\nHybridoma: \nSpleen cells from immunized BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line. \n\nRecommended Secondary Antibodies:\nI1904-65W: IgG, F(ab

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SPECIFICATIONS

Catalog Number

V0500-00F

Size

100ug

Applications

ELISA, IHC, IP, WB

Hosts

Mouse

Form

Supplied as a liquid in 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Southern, J.A. et al. (1991) Identification of an epitope on the P & V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. J. Gen. Virol. 72: 1551-1557. 2. Hanke, T. et al. (1992) Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. J. Gen. Virol. 73: 653-660. 3. Randall, R.E. et al. (1993) Two-tag purification of recombinant proteins for the construction of solid matrix- antibody antigen (SMAA) complexes as vaccines. Vaccine 11: 1247-1252. 4. Randall, R.E. et al. (1994) Purification of antibody- antigen complexes containing recombinant SIV proteins; comparison of antigen and antibody-antigen complexes for immune priming. Vaccine 12: 351-358. 5. Hanke, T. et al. (1995) Attachment of an oligopeptide epitope to the C-terminus of recombinant SIV gp160 \nfacilitates the construction of SMAA complexes while preserving CD4 binding. J. Virol. Methods 53: 149-156. 6. Jaffray, E. et al. (1995) Domain organization of I kappa B alpha and sites of interaction with NF-kappa B p65 Mol. Cell. Biol. 15: 2166-2172. 7. Rodriguez, M.S. et al. (1995) Inducible degradation of IkB in vitro and in vivo requires the acidic C-terminal domain of the protein. Mol. Cell. Biol. 15: 2413-2419. 8. Arenzana-Seisdedos, F. et al. (1995) Inducible nuclear expression of newly synthesised IkBa negatively regulates DNA binding and transcriptional activities of NF-kB. Mol. Cell. Biol. 15: 2689-2696. 9. Hirst, K. et al. (1994) The transcription factor, the Cdk, its cyclin and their regulator: directing the transcriptional \nresponse to a nutritional signal EMBO J. 13: 5410-5420. 10. Dunn, C. et al. (1999) Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag. \nJ. Immunol. Methods. 244: 141-150. 11. Young, D.F. et al. (2001) Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells J. Virol. 75: 3363 - 3370.

Additional Info

Recognizes recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. (V5-Tag). Detects recombinant proteins including transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr.

Alternative Names

PK-TAG