VAP-1 (Vascular Adhesion Protein 1, Amine Oxidase Copper-Containing 3, AOC3, Semicarbazide-Sensitive Amine Oxidase, SSAO)

Vascular adhesion protein 1 (VAP-1), also known as AOC3 (amine oxidase copper-containing 3) or SSAO (semicarbazide-sensitive amine oxidase) is a 170 kD type II transmembrane glycoprotein consisting of two disulfide-linked 763 amino acid (aa) subunits. It is expressed on endothelium, vascular smooth muscle, and adipocytes. The extracellular domain mediates lymphocyte rolling, adhesion, possibly extravasations during inflammation, and possesses monoamine oxidase activity. Adipocytes can release a soluble form via metalloproteinase cleavage. Human VAP-1 ECD shares 83% and 88% aa sequence identity with mouse and canine VAP-1 ECD, respectively.\n\nApplications: \nWestern Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human VAP-1. The detection limit for rhVAP-1 is approximately 25 ng/lane under non-reducing and reducing conditions. \nDirect ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human VAP-1. The detection limit for rhVAP-1 is approximately 0.5 ng/well. In this format, this antibody shows approximately 50% cross-reactivity with rmVAP-1. \nOptimal dilutions should be determined by each laboratory for each application.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

V2095-03

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.

P Type

Pab

Purity

Purified by human VAP-1 affinity chromatography.

Isotype

IgG

Additional Info

Selected for its ability to recognize human VAP-1 in direct ELISAs and Western Blots.