VAP-1 is present in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. The majority of VAP-1 is stored within intracellular granules and translocated to the endothelial surface on inflammation. Structurally it belongs to enzymes called semicarbamizide-sensitive amine oxidases. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, aldehyde, and ammonia. VAP-1 possesses both adhesive and enzymatic properties in vitro. VAP-1 mediates leukocyte trafficking to sites of inflammation.
Applications:
Suitable for use in Immunohistochemistry, Immunoprecipitation, Immunofluorescence, Flow Cytometry and Inhibition of biological activity. Other applications have not been tested.
Recommended Dilution:
Immunohistochemistry (frozen): 1:50. Tissue sections were fixed in acetone and incubated with antibody for 20 min at RT.
Flow Cytometry: 1:50. Stains the extracellular domain of mouse VAP-1 in CHO cells transfected with mouse VAP-1 domain.
Optimal dilutions to be determined by the researcher.
Positive Control:
Mouse VAP-1-transfected CHO cells
Negative Control:
Mock-transfected CHO cells
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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