VAP-1 (Vascular Adhesion Protein 1, VAP1, Amine Oxidase Copper-containing 3 (Vascular Adhesion Protein 1), AOC3, Copper Amine Oxidase, HPAO, Membrane Copper Amine Oxidase, Semicarbazide-sensitive Amine Oxidase, SSAO, VP97)

VAP-1 is present in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. The majority of VAP-1 is stored within intracellular granules and translocated to the endothelial surface on inflammation. Structurally it belongs to enzymes called semicarbamizide-sensitive amine oxidases. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, aldehyde, and ammonia. VAP-1 possesses both adhesive and enzymatic properties in vitro. VAP-1 mediates leukocyte trafficking to sites of inflammation.\n\nApplications:\nSuitable for use in Immunohistochemistry, Immunoprecipitation, Immunofluorescence, Flow Cytometry and Inhibition of biological activity. Other applications have not been tested. \n\nRecommended Dilution:\nImmunohistochemistry (frozen): 1:50. Tissue sections were fixed in acetone and incubated with antibody for 20 min at RT.\nFlow Cytometry: 1:50. Stains the extracellular domain of mouse VAP-1 in CHO cells transfected with mouse VAP-1 domain.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nMouse VAP-1-transfected CHO cells\n\nNegative Control: \nMock-transfected CHO cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2095-02C

Size

100ug

Applications

FC, IF, IHC, IP

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1.-Abella, A et al; Adipocytes release a soluble form of VAP-1/SSAO by a metalloprotease-dependent process and in a regulated manner. Diabetologia 2004, 47: 429 2.-Merinen, M et al; Vascular adhesion protein-1 is involved in both acute and chronic inflammation in the mouse. Am J Path 2005, 166: 793 3.-Bonder, C et al; Rules of recruitment for Th1 and Th2 lymphocytes in inflamed liver: a role for alpha-4 integrin and vascular adhesion protein-1. Immunity 2005, 23: 153 4.-Marttila-Ichihara, F et al; Small-molecule inhibitors of vascular adhesion protein-1 reduce the accumulation of myeloid cells into tumors and attenuate tumor growth in mice. J Immunol 2010, 184: 3164 5.-Iffiú-Soltész, Z et al; Increased primary amine oxidase expression and activity in white adipose tissue of obese and diabetic db-/- mice. J Neural Transm 2011, DOI: 10.1007/s00702-011-0586-9

Additional Info

Recognizes mouse Vascular Adhesion Protein-1 (VAP-1), also known as AOC3, a heavily sialylated homodimeric glycoprotein of 180kD. Inhibits migration of granulocytes and monocytes in acute models of inflammation.

Alternative Names

SSAO, Semicarbazide-sensitive amine oxidase