Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr1054) (VEGF R2, Flk1, KDR, Receptor Tyrosine Kinase)

Vascular endothelial growth factor receptor 2 (VEGFR2, also known as KDR/FLK-1) is a 200kD member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation migration and differentiation. Like other growth factor receptors upon ligand binding, VEGFR2 forms a dimer and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2- and phosphotyrosine bindingcontaining signaling proteins. Phosphorylation of tyrosines 1054 and 1059 in the activation loop is required for activation of VEGFR2 and its intrinsic tyrosine kinase activity. \n\nApplications: \nSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.35-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nNIH3T3 cells transfected with full-length, wild type VEGFR2, or Porcine Aortic Epithelial (PAE) cells transfected with a chimeric receptor consisting of the extracellular domain of the CSF-1 receptor coupled to the transmembrane and cytoplasmic domains of the VEGFR2.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2110-19C

Size

10 Blots

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Meyer, R.D., et al. (2002) The presence of a single tyrosine residue at the carboxyl domain of vascular endothelial growth factor receptor-2/FLK-1 regulates its autophosphorylation and activation of signaling molecules. J. Biol. Chem. 277(30):27081-27087. 2. Sato, Y., et al. (2000) Properties of two VEGF receptors, Flt-1 and KDR, in signal transduction. Ann. N.Y. Acad. Sci. 902:201-207. 3. Rahimi, N. and A. Kazlauskas (1999) A role for cadherin-5 in regulation of vascular endothelial growth factor receptor 2 activity in endothelial cells. Mol. Biol. Cell 10(10):3401-3407.

Additional Info

Recognizes mouse VEGFR2. Species sequence homology: Human and rat VEGFR3 (100%).