Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr951) (VEGF R2, Flk1)

Vascular endothelial growth factor receptor 2 (VEGFR-2, KDR, Flk-1) is a major receptor transducing the effects of VEGF into endothelial cells. Upon binding to specific ligands, VEGFR-2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR-2 are located in the\nkinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of the adaptor proteins, including Shc, Grb-2, PI3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The signaling of VEGFR-2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and prouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (4).\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2110-18G

Size

100ul

Applications

WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 100ug/ml BSA, 50% glycerol, < 0.02% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

(1) Meyer, M. et al. (1999) EMBO J. 18, 363

Additional Info

Detects overexpressed levels of VEGF receptor 2 only when phosphorylated at tyrosine 951. MW: 180kD. Does not crossreact with other tyrosine-phosphorylated proteins. Species Crossreactivity: Mouse. Predicted to react with human based on 100% sequence homology.