Vascular Endothelial Growth Factor Receptor 3 (VEGF 3, Flt4, MYYN)

Applications: \nSuitable for use in Immunohistochemistry, Westerm blot, and Immunocytochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry: 1:100-1:400 High temperature antigen retrieval in 10mM citrate buffer (pH6.0) is strongly recommended. Blocking with 2% normal serum plus 1% BSA in PBS is suggested. Primary antibody should be diluted in PBS with 0.1% BSA only. Triton X-100 or excess blocking compounds is not recommended.\nWestern Blot: Membrane extract preparations and the liberal use of proteinase inhibitors is strongly recommended.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nMCF7, SW480 and MDA-MB cell lines are positive controls for Immunocytochemistry.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts