Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF)

Applications: \nSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry (frozen and paraffin-embedded sections). Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 1:20-1:50 using ABC method. We recommend incubation period of 30-60 minutes at RT. High temperature treatment of formalin-fixed tissue sections using 1mM EDTA, pH 8.0 must be performed prior to immunostaining.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2110-08A

Size

100ug

Applications

IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Giatromanolaki A et al. DEC1 (STRA13) protein expression relates to hypoxia- inducible factor 1-alpha and carbonic anhydrase-9 overexpression in non-small cell lung cancer. J Pathol 200:222-8 (2003). PubMed: 12754744\n2. Turley H et al. Expression of VEGF in routinely fixed material using a new monoclonal antibody VG1. J Pathol 186:313-8 (1998). PubMed: 10211122

Additional Info

Recognizes the 189, 165 and 121 isoforms of VEGF. Species Crossreactivity: human, mouse, rat and canine.