Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). Study of tumor angiogenesis has led to the identification of several proteins including basic fibroblast growth factor (bFGF) and vascular endothelial growth factor. VEGF acts by interacting with a family of largely endothelial-specific receptor tyrosine kinases that includes VEGFR1 (flt-1/flk-2), VEGFR2 (flk- 1/KDR), and VEGFR3/Flt-4. Disruption of VEGFRs interferes with differentiation of endothelial cells and it is lethal for the embryo. VEGFR3 (mouse 1363aa and human 1298aa), a receptor protein tyrosine kinase, is a receptor for VEGF-C. Mouse VEGFR3 protein topology: 1-24aa signal peptide, 25-1363 mature protein (25-775 EC domain, 776-797aa TM domain, 798-1363aa CP domain). VEGFR3 is type 1 membrane protein. It is expressed in adult lung, and liver, and in fetal liver, brain, intestine, and placenta.
Applications:
Suitable for use in Western Blot, ELISA, and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
Western Blot: 1-10ug/ml (ECL)
ELISA: 1:10,000-1:100,000; 50-100ng of control peptide/well
Immunohistochemistry: 2-20ug/ml in formaldehyde fixed tissues
Optimal dilutions to be determined by the researcher.
Control Peptide:
F4601-03: Flt4, Mouse, Control Peptide (Fms-like Tyrosine Kinase 4)
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.