Vimentin (VIM)

Cat no: V2122-14D

Supplier: United States Biological

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Vimentin is an intermediate filament protein found in cells of mesenchymal origin such as endothelial cells, fibroblasts, smooth muscle cells and lymphoid cells. This antibody will help detect vimentin and cells of mes- enchymal origin in normal and uncharacterized tissues. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:25-1:50 Immunohistochemistry: 1:50-1:100 for 1 hr. at RT Optimal dilutions to be determined by the researcher. Positive Control: Western Blot: Skin Immunohistochemistry: Tonsil, lymph node. Cytoplasmic staining pattern. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	V2122-14D
Reactivities:	Human, Rat, Canine, Chicken/Bird, Feline, Non-Human Primate
Hosts:	Mouse
Applications:	Immunohistochemistry, Western Blot
Size:	1ml
Form:	Supplied as a liquid in 15mM sodium azide.
P type:	Mab
Purity:	Supernatant
References:	1.Sheibani K and Battifora H. Signet ring cell melanoma. A rare morphologic variant of malignant melanoma. Am. J. Surg. Pathol. 12:28-34 (1988). 2.Azumi N and Battifora H. The distribution of vimentin and keratin in epithelial and nonepithelial neoplasms. A comprehensive immunohistochemical study on formalin- and alcohol-fixed tumours. Am. J. Clin. Pathol. 88:286-96 (1987). 3.Benjamin E, Law S and Bobrow L G. Intermediate filaments cytokeratin and vimentin in ovarian sex cordstromal tumours with correlative studies in adult and fetal tissues. J. Pathol. 152:253-63 (1987). 4.Miettinen M, Lehto V P and Virtanen I. Antibodies to intermediate filament proteins. The differential diagnosis of cutaneous tumors. Arch. Dermatol. 121:736-41 (1985). 5.Osborn M, Debus E and Weber K. Monoclonal antibodies specific for vimentin. Eur. J. Cell Biol. 34:137-43 (1984).
Additional info:	Recognizes Vimentin. Species Crossreactivity: Canine, chicken, equine, feline, human, monkey and rat.

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Vimentin (VIM)

Cat no: V2122-14D

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