Vimentin (VIM)

Vimentin is an intermediate filament protein found in cells of mesenchymal origin such as endothelial cells, fibroblasts, smooth muscle cells and lymphoid cells. This antibody will help detect vimentin and cells of mes- enchymal origin in normal and uncharacterized tissues.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:25-1:50\nImmunohistochemistry: 1:50-1:100 for 1 hr. at RT\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nWestern Blot: Skin\nImmunohistochemistry: Tonsil, lymph node. Cytoplasmic staining pattern.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2122-14D

Size

1ml

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Rat, Can, Ch/Bird, Fel, NHP

Form

Supplied as a liquid in 15mM sodium azide.

P Type

Mab

Purity

Supernatant

References

1.Sheibani K and Battifora H. Signet ring cell melanoma. A rare morphologic variant of malignant melanoma. Am. J. Surg. Pathol. 12:28-34 (1988). 2.Azumi N and Battifora H. The distribution of vimentin and keratin in epithelial and nonepithelial neoplasms. A comprehensive immunohistochemical study on formalin- and alcohol-fixed tumours. Am. J. Clin. Pathol. 88:286-96 (1987). 3.Benjamin E, Law S and Bobrow L G. Intermediate filaments cytokeratin and vimentin in ovarian sex cordstromal tumours with correlative studies in adult and fetal tissues. J. Pathol. 152:253-63 (1987). 4.Miettinen M, Lehto V P and Virtanen I. Antibodies to intermediate filament proteins. The differential diagnosis of cutaneous tumors. Arch. Dermatol. 121:736-41 (1985). 5.Osborn M, Debus E and Weber K. Monoclonal antibodies specific for vimentin. Eur. J. Cell Biol. 34:137-43 (1984).

Additional Info

Recognizes Vimentin. Species Crossreactivity: Canine, chicken, equine, feline, human, monkey and rat.