Vinculin (VIN-1)

Vinculin, a cytoskeletal protein found in adherens type cell-cell and cell-extracellular matrix junctions, displays considerable heterogeneity. At least three 117kD and two 130kD isoforms (muscle specific metavinculin) have been detected. Vinculin binds talin and a-actinin in adhesion plaques and may play either a structural or a regulatory role in these structures.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-1ug/ml detects Vinculin in RIPA lysates of human A431 cells; previous lots detected Vinculin in RIPA lysates from chicken embryonic fibroblasts (CEF) and mouse 3T3 fibroblasts. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with V2122-50 (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. \nImmunoprecipitation: 4ug immunoprecipitates Vinculin from mouse 3T3/A31 and human A431 RIPA lysates. \nImmunohistochemistry and Immunocytochemistry (frozen and formalin fixed sections): 10ug/ml will stain Vinculin in Ref-52 cells fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100. \nOptimal dilutions to be determined by the researcher. \n\nPositive Antigen Control: A0001-50, Non-stimulated A431 cell lysate. Use 20ug per lane for minigels. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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