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ZO-1 (Mid) (Tight Junction-associated Polypeptide, Zonula Occludens 1)

Cat no: Z0750-02D

ZO-1 (Mid) (Tight Junction-associated Polypeptide, Zonula Occludens 1)

The tight junction is a cell-to-cell adhesion structure in epithelial cells that constitute the epithelial junctional complex with adherens junctions and desmosomes. Tight junctions seal cells to create a primary barrier to the diffusion of solutes across the cellular sheet and also function as a boundary between the apical and basolateral membrane domains to produce their polarization.1 Tight junction strands are mainly composed of claudins, occludin, and JAM.2 Various scaffold proteins have been reported to be concentrated at the cytoplasmic surfaces of the junctional complex regions to determine the specialization and localization of junctions: ZO-1, ZO-2, and ZO-3. The zona occludins (ZO) proteins constitute the plaque structures underlying plasma membranes together with various proteins including cingulin, symplekin, Par-3

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SPECIFICATIONS

Catalog Number

Z0750-02D

Size

100ug

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Anderson JM and Van Itallie CM. 1995. Am J Physiol 269:G467-G475. 2. Umeda K, et al. 2004. J Biol Chem. 279(43):44785-44794. 3. Itoh M, et al. 1993. J Cell Biol. 121:491-502. 4. Martin TA, et al. 2004. Eur J Cancer. 40(18):2717-2725. 5. Billings SD, et al. 2004. Mod Pathol. 17(2):141-149.

Additional Info

Recognizes human ZO-1 protein at ~225kD. Bands of unknown origin may be observed at lower molecular weights in some sample lysates. These bands may represent breakdown products.

Read more on Supplier website

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum

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